Pt. I Assessing Risk of Infection and Severity of Disease -- Ch.1 Vector Incrimination and Entomological -- Ch.2 Epidemiological Measures of Risk of Malaria / J. Kevin Baird, Michael J. Bangs, Jason D. Maguire 13 Pt. II Laboratory Models-- Ch.3 Maintenance of the Plasmodium berghei Life Cycle / Robert E. Sinden, Geoff A. Butcher, A. L. Beetsma -- Ch.4 Mouse Models for Pre-Erythrocytic-Stage Malaria / Laurent Renia, Elodie Belnoue, Irene Landau -- Ch.5 Mouse Models for Erythrocytic-Stage Malaria / Latifu A. Sanni, Luis F. Fonseca, Jean Langhorne -- Ch.6 Nonhuman Primate Models: I. Nonhuman Primate Host-Parasite Combinations / William E. Collins -- Ch. 7 Nonhuman Primate Models: II. Infection of Saimiri and Aotus Monkeys with Plasmodium vivax / William E. Collins-- Pt. III Diagnosis and Typing -- Ch.8 Vector Analysis / John C. Beier -- Ch.9 Genotyping of Plasmodium spp.: Nested PCR / Georges Snounou -- Ch.10 Genotyping of Plasmodium falciparum: PCR-RFLP Analysis / Ingrid Felger, Hans-Peter Beck -- Ch.1 Microsatellite Analysis in Plasmodium falciparum / Xin-zhuan Su, Michael T. Ferdig -- Ch.12 Quantitation of Liver-Stage Parasites by Automated TaqMan Real-Time PCR / Adam A. Witney, Robert M. Anthony, Daniel J. Carucci -- Ch.13 Quantitation of Liver-Stage Parasites by Competitive RT-PCR / Kyle C. McKenna, Marcelo R. S. Briones -- Pt. IV Molecular Biology Techniques -- Ch.14 Extraction and Purification of Plasmodium Total RNA / Till Voss -- Ch.15 Extraction and Purification of Plasmodium Parasite DNA / Hans-Peter Beck -- Ch.16 Southern Blotting of Parasite DNA / Tobias Spielmann -- Ch.17 SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins / Roland A. Cooper -- Ch.18 Nested PCR Analysis of Plasmodium Parasites / Georges Snounou, Balbir Singh -- Ch.19 RFLP Analysis / Hans-Peter Beck -- Ch.20 Analysis of Gene Expression by RT-PCR / Peter Preiser -- Ch.21 In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites / Joanne Thompson -- Ch. 22 Purification of Chromosomes from Plasmodium falciparum / Daniel J. Carucci, Paul Horrocks, Malcolm J. Gardner -- Ch.23 Construction of Genomic Libraries from the DNA of Plasmodium Species / Leda M. Cummings, Dharmendar Rathore, Thomas F. McCutchan -- Ch.24 Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA / Dharmendar Rathore, Thomas F. McCutchan -- Ch.25 Construction of Plasmodium falciparum [lambda] cDNA Libraries / David A. Fidock, Dharmendar Rathore, Thomas F. McCutchan -- Ch.26 Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization / David A. Fidock, Thanh V. Nguyen, Brenda T. Beerntsen -- Ch.27 Construction and Screening of YAC Libraries / Cecilia P. Sanchez, Martin Preuss, Michael Lanzer -- Ch.28 Episomal Transformation of Plasmodium berghei / Chris J. Janse, Andrew P. Waters -- Ch.29 Gene Targeting in Plasmodium berghei / Vandana Thathy, Robert Menard -- Pt. V Immunological Techniques -- Ch.30 Peptide Vaccination / Valentin Meraldi, Jackeline F. Romero, Giampietro Corradin -- Ch. 31 DNA Vaccination / Richard C. Hedstrom, Denise L. Doolan -- Ch.32 Assessing Antigen-Specific CD8[superscript +] and CD4[superscript +] T-Cell Responses in Mice After Immunization with Recombinant Viruses / Moriya Tsuji -- Ch.33 Assessing CD4[superscript +] Helper T-Lymphocyte Responses by Lymphoproliferation / Isabella A. Quakyi, Jeffrey D. Ahlers -- Ch.34 Limiting Dilution Analysis of Antigen-Specific CD4[superscript +] T-Cell Responses in Mice / Elsa Seixas, Jean Langhorne, Stuart Quin -- Ch. 35 Cell Trafficking: Malaria Blood-Stage Parasite-Specific CD4[superscript +] T Cells After Adoptive Transfer into Mice / Chakrit Hirunpetcharat, Michael F. Good 401 36 Assessing Antigen-Specific Proliferation and Cytokine Responses Using Flow Cytometry / Catherine E. M. Allsopp, Jean Langhorne -- Ch.37 Cytokine Analysis by Intracellular Staining / Aftab A. Ansari, Ann E. Mayne -- Ch. 38 Assessment of Antigen-Specific CTL- and CD8[superscript +]-Dependent IFN-[gamma] Responses in Mice / Katrin Peter, Regine Audran, Anilza Bonelo -- Ch.39 Assessing Antigen-Specific CD8[superscript +] CTL Responses in Humans / Denise L. Doolan -- Ch.40 Human Antibody Subclass ELISA / Pierre Druilhe, Hasnaa Bouharoun-Tayoun -- Ch.41 Systemic Nitric Oxide Production in Human Malaria: I. Analysis of NO Metabolites in Biological Fluids / Nicholas M. Anstey, Craig S. Boutlis, Jocelyn R. Saunders -- Ch. 42 Systemic Nitric Oxide Production in Human Malaria: II. Analysis of Mononuclear Cell Nitric Oxide Synthase Type 2 Antigen Expression / Jocelyn R. Saunders, Mary A. Misukonis, J. Brice Weinberg -- Pt. VI Cell Biology Techniques 43 In Vitro Culture of Plasmodium Parasites / James B. Jensen -- Ch. 44 Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator / J. David Haynes, J. Kathleen Moch -- Ch. 45 Hepatic-Portal Branch Inoculation / John B. Sacci, Jr. -- Ch. 46 Hepatocyte Perfusion, Isolation, and Culture / John B. Sacci, Jr. -- Ch.47 Inhibition of Sporozoite Invasion: The Double-Staining Assay / Laurent Renia, Ana Margarida Vigario, Elodie Belnoue -- Ch.48 Inhibition of Liver-Stage Development Assay / John B. Sacci, Jr. -- Ch. 49 T-Cell Mediated Inhibition of Liver-Stage Development Assay / Laurent Renia, Elodie Belnoue, Ana Margarida Vigario -- Ch. 50 Antibody-Dependent Cellular Inhibition Assay / Pierre Druilhe, Hasnaa Bouharoun-Tayoun -- Ch. 51 Erythrocytic Malaria Growth or Invasion Inhibition Assays with Emphasis on Suspension Culture GIA / J. David Haynes, J. Kathleen Moch, Douglas S. Smoot -- Ch.52 Analysis of CSA-Binding Parasites and Antiadhesion Antibodies / Michal Fried, Patrick E. Duffy -- Ch.53 Analysis of the Adhesive Properties of Plasmodium falciparum-Infected Red Blood Cells Under Conditions of Flow / Brian M. Cooke, Ross L. Coppel, Gerard B. Nash -- Ch.54 Preparation of Adhesive Targets for Flow-Based Cytoadhesion Assays / Brian M. Cooke, Ross L. Coppel, Gerard B. Nash -- Ch.55 Triton X-114 Phase Partitioning for Antigen Characterization / Lina Wang, Ross L. Coppel -- Ch.56 Immunoprecipitation for Antigen Localization / John G. T. Menting, Ross L. Coppel -- Pt. VII Testing Interventions For Malaria Control -- Ch.57 Field Trials / Pedro L. Alonso, John J. Aponte

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